README for Figure 4B.csv 
*** This file contains the raw data obtained on DNA and DNA-MJ1647 complexes using tethered particle motion represented in Figure 4B of 
Article: DNA-bridging by an archaeal histone variant via a unique tetramerisation interface 
Authors: Ofer, Blombach, Erkelens, Barker, Schwab, Smollett, Matelska, Fouqueau, van der Vis, Kent, Pinotsis, Dame and Werner
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl and f.werner@ucl.ac.uk

Legend Figure 4:MJ1647 tetramers compact and bridge DNA strands. 
(A-C) Tethered Particle Motion (TPM) experiments testing DNA compaction by A3 (G), MJ1647 (H) and MJ1647C (I). Root Mean Square displacement (RMS) values are plotted as a function of histone concentration. The RMS values were obtained from fitting the data with a Gaussian distribution. Error bars represent the propagated standard deviation from at least two individual measurements. Dashed line is a line to guide the eye. For MJ1647, the roman numbers (I, II and III) represent three populations at ~150, 132 and 112 nm at 36 nM MJ1647 used for end-to-end distance calculations (see panel D).
(D) Histograms of calculated end-to-end distances of the three populations observed at 36 nM MJ1647. Of each population, the 25 beads closed to the fitted RMS value were selected and the end-to-end distance was calculated of the 2.5% most distant positions with respect to the center of the beads. Histograms were fit with a skewed normal distribution. Insert: pairwise distribution plot of the differences between the end-to-end distance peaks I, II and III. Histograms were fitted with a Gaussian distribution.
(E) DNA bridging assays. DNA recovery (%) is plotted as a function of protein concentration. Data are plotted as mean values of three independent measurements and error bars represent the standard deviation. Dashed line is a line to guide the eye. 


 *** The data were obtained using Tethered particle motion as described in the associated article. 

*** Data obtained for each protein concentration(s) is given in this table as plotted in figure 4B.
Column A: MJ1647 (nM) = Concentration of MJ1647 in nanomolar
Column B: Replicate = number of replicate
Column C: RMS (nm) = Root mean square deviation in nanometer measured for each bead 
Column D: rho = anisotropic ratio (movement in x-direction/movement in y-direction) 
Column E: stdev = standard deviation 
Column F: FoV = field of view (data with the same FoV were collected in one image) 
Column G: bead = beads in each FoV are represented by a unique number 
The values given in column D and E are tether quality criteria and used for data filtering as described in the associated article.